Experimental Characterization of a Microfluidic Device Based on Passive Crossflow Filters for Blood Fractionation

The separation of red blood cells (RBCs) from plasma and the analysis individual RBCs are great importance, as they provide valuable information regarding health their donor. Recent developments in microfluidics microfabrication have contributed to fabrication microsystems with complex features promote RBCs. In this work, capacity a multi-step crossflow microfluidic device was evaluated by using analogue fluid made Brij L4 micelles human separated whole blood, suspended solution hematocrits (Ht) 0.5 1%. All samples collected at outlets were experimentally analyzed compared. absorbance spectrum also measured for prepared samples. results indicate that tested has exhibited flow behavior similar blood. addition, optical spectrophotometry revealed it possible evaluate efficiency device, concluding concentration lower most lateral outside microchannel due cumulative effect multiple cross-flow filters.